A TaqI polymorphism in the human cyclin A gene
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چکیده
منابع مشابه
A TaqI polymorphism in the human cyclin A gene.
PCR Conditions: The PCR reaction is carried out in a total volume of 50 /*1 containing approximately 250 ng DNA, 2 units Taq DNA polymerase, 50 pmol of each primer, 200 /M dNTP's, 10 mM Tris-HCl pH 8.3, 50 mM KC1, 1.5 mM MgCl2, 0.001% gelatin. The amplification is performed for 30 cycles with an annealing temperature of 60°C. The amplified product is digested with TaqI and the DNA fragments are...
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SOURCE AND DESCRIPTION : The probe that contains exon 1 to 8 was generated from pLDLR3. pLDLR3 contains a full-length LDL receptor cDNA (Yamamoto et at. , 1984) POLYMORPHISM : TaqI identifies a bi-allelic polymorphism with a band of 1.7kb and 0.6kb. FREQUENCY : Studies in 55 unrelated normal Japanese. 1.7Vb fragment 0.69 0.6kb fragment 0.31 NOT POLYMORPHISMS : In 50 individuals no polymorphisms...
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References: l)Nussbaum,R.L., Airhart,S.D. and Ledbetter.D.H. (1986) Am. J. Med. Genet. 23, 457-466. 2)Patterson,M.N., Bell,M.V., BloomfieldJ,, Flint,T., Dorkins.H., Thibodeau.S.N., Schaid,D., Bren,G., Schwartz,C.E., Wieringa,B., Ropers,H.-H., Callen.D.F., Sutherland.G., Froster-Iskenius.U., Vissing.H. and Davies,K.E. (1989) Genomics 4, 570-578. A Taql polymorphism adjacent to the factor VIII ge...
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Altered response to growth rate changes in Kluyveromyces lactis versus Saccharomyces cerevisiae as demonstrated by heterologous expression of ribosomal protein 59 {CRY1) Figures 5 and 6 of this article were inadvertantly transposed. The legend to figure 5 should accompany figure 6 as labelled and the legend to figure 6 should accompany figure 5.
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.9.2516